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I.R.F. / Aging news / Diseases / 10112901

Fetal Liver Hepatocytes in Recovery of Liver Function
Posted on: November 29, 2010

The use of cell transplantation as an alternative therapy for orthotopic liver transplantation has been widely anticipated due to a chronic donor shortage. In this study, scientists transplanted HPCs into the liver injury model mice to determine whether HPC transplantation may improve the liver dysfunction.

The scientists obtained donor cells from E13.5 fetal livers of green fluorescent protein (GFP) transgenic mice and transplanted GFP-positive fetal liver cells into the transgenic mice which express diphtheria toxin (DT) receptors under the control of an albumin enhancer/promoter. Subsequently, they induced selective liver injury to recipient mice by DT administration and then evaluated the engraftment of the transplanted cells and their effect on survivorship.

They found that low dose of DT induced sublethal liver injury and the high dose of DT was lethal to the liver injury model mice. The transplanted GFP-positive cells were engrafted into the recipient livers and expressed albumin, resembling mature hepatocytes. They continued to proliferate, forming clusters. The survival rate at 25 days after transplantation of the cell-transplanted group (8 of 20; 40.0%) was improved significantly (P=0.0047) in comparison to that of the sham-operated group (0 of 20; 0%).

Briefly it can be concluded that transplanted cells were engrafted and repopulated the liver of recipient mice, resulting in the improvement of the survival rate of the liver injury model mice. It could be proposed that HPCs are a desirable cell source for cell transplantation.

Source: Machimoto T, Yasuchika K, Komori J, Ishii T, Kamo N, Shimoda M, Konishi S, Saito M, Kohno K, Uemoto S, Ikai I.; Improvement of the survival rate by fetal liver cell transplantation in a mice lethal liver failure model.; Transplantation. 2007 Nov 27;84(10):1233-9.
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